Last data update: 24 January 2024 16:39 CET
Host name: Escherichia coli K12 W3110 Falcon (LMBP 13411)
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| Species: | Escherichia coli |
| Group: | K12 |
| Strain designation: | W3110 Falcon |
| Accession number: | LMBP 13411 |
| Depositor: | This host strain was deposited by Prof. Dr B. Wren1. It was constructed by Dr E. Kay1. 1 Department of Infection Biology, London School of Hygiene and Tropical Medicine, London, UK |
| Containment level: | This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms, and its updates (see also the Belgian risk group classification). |
| Other culture collection numbers: | - |
| Medium: | LB-Lennox |
| Selection marker: | / |
| Temperature: | 37°C |
| Genotype: | F- λ- IN(rrnD-rrnE)1 rph-1 ΔlpxM ΔwecA-wzzE(gne) (Source: Kay et al. (2022)) |
| Phenotype: | - |
| Properties: | This host strain is a derivative of the Raptor strain and can be used for bioengineering where purified products with reduced endotoxicity are desirable. Enterobacterial common antigen is no longer produced and a UDP N-acetylglucose epimerase has been chromosomally integrated increasing the availability of UDP-GalNAc. The genes wecA-wzzE in the ECA synthesis locus were replaced with gne, UDP N-acetylglucose epimerase from the Campylobacter jejuni strain 81116, which was inserted under control of the native, constitutive wecA promoter, in the Raptor background using CRISPR technology. wzzE encodes the enterobacterial common antigen chain length regulator which was removed to prevent any interference with recombinant polysaccharide production. |
| Further information: | This strain is part of a versatile suite of glycoengineering strains for various downstream applications, such as glycoconjugate vaccine production. |
| Original reference: | Kay et al., Microb. Cell Fact. 21 (2022), 66 [PMID: 35449016] [DOI: 10.1186/s12934-022-01792-7] |
| Restricted use: | - BCCM MTA - The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC. |
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Refer in your Materials and Methods: |
Escherichia coli K12 W3110 Falcon (LMBP 13411) is available at BCCM/GeneCorner. It was deposited by Prof. Dr B. Wren and was published in Kay et al., 2022. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.